Distinct Allosteric Networks Underlie Mechanistic Speciation of ABC Transporters

Burcin Acar, Jessica Rose, Burcu Aykac Fas, Nir Ben-Tal, Oded Lewinson, and Turkan Haliloglu

Structure. 2020, June, 28; 1–13


Substrate recognition and ATPase activity of the E. coli cysteine/cystine ABC transporter YecSC-FliY

Siwar Sabrialabed, Janet G. Yang, Elon Yariv, Nir Ben-Tal, and Oded Lewinson

J. Biol. Chem. 2020 April, 295(16); 5245–5256



Vitamin B12 import is all about timing

News and view by Lutz Schmitt Nature Chem Biology. 2018  July, 14; 640–641


Single-molecule probing of the conformational homogeneity of the ABC transporter BtuCD

Min Yang, Nurit Livnat Levanon, Burçin Acar, Burcu Aykac Fas, Gal Masrati, Jessica Rose, Nir Ben-Tal, Turkan Haliloglu, Yongfang Zhao, and Oded Lewinson. Nature Chem Biology. 2018  July, 14; 715–722



ATP binding and hydrolysis disrupts the high-affinity interaction between the heme ABC transporter HmuUV and its cognate substrate binding protein

Hiba Qasem Abdullah, Michal Perach, Nurit Livnat-Levanon, Oded Lewinson.

J Biol Chem. 2017 Jul 14. pii: jbc. M117.779975


The highly synergistic, broad spectrum, antibacterial activity of organic acids and transition metals

Daniel Zhitnitsky, Jessica Rose, Oded Lewinson.

 Sci Rep. 2017 Mar 15;7:44554


Mechanism of Action of ABC Importers: Conservation, Divergence, and Physiological Adaptations

Oded Lewinson, Nurit Livnat-Levanon. J Mol Biol. 2017 Mar 10;429(5):606-619


L-glutamine Induces Expression of Listeria monocytogenes Virulence Genes

Adi Haber, Sivan Friedman, Lior Lobel, Tamar Burg-Golani, Nadejda Sigal, Jessica Rose, Nurit Livnat-Levanon, Oded Lewinson, Anat A. Herskovits. PLoS Pathog. 2017 Jan 23;13(1):e1006161


The uncoupled ATPase activity of the ABC transporter BtuC2D2 leads to a hysteretic conformational change, conformational memory, and improved activity

Nurit Livnat-Levanon, Amy I. Gilson, Nir Ben-Tal, Oded Lewinson. Sci Rep. 2016 Feb 22;6:21696



Metal binding spectrum and model structure of the Bacillus anthracis virulence determinant MntA

Elena Vigonsky, Inbar Fish, Nurit Livnat-Levanon, Elena Ovcharenko, Nir Ben-Tal, Oded Lewinson. Metallomics. 2015 Oct;7(10):1407-19


Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Nurit Livnat Levanon, Elena Vigonsky, Oded Lewinson. J Vis Exp. 2014 Nov 29;(93):e51937


Structural Basis for Heavy Metal Detoxification by an Atm1-Type ABC Exporter

Jonas Y Lee, Janet G Yang, Daniel Zhitnitsky, Oded Lewinson, Douglas C Rees. Science 7 March 2014: Vol. 343 no. 6175 pp. 1133-1136 


Identification of functionally important conserved trans-membrane residues of bacterial PIB-type ATPases

Daniel Zhitnitsky, Oded Lewinson. Molecular Microbiology Volume 91, Issue 4, pages 777–789, February 2014


A Relay Network of Extracellular Heme-Binding Proteins Drives C. albicans Iron Acquisition from Hemoglobin

Kuznets G, Vigonsky E, Weissman Z, Lalli D, Gildor T, Kauffman SJ, Turano P, Becker J, Lewinson O, Kornitzer D. PLoS Pathogens Oct 2, 2014



Two molybdate/tungstate ABC transporters that interact very differently with their substrate binding proteins

Elena Vigonsky, Elena Ovcharenko, Oded Lewinson. Proc Natl Acad Sci U S A. 2013



A distinct mechanism for the ABC transporter BtuCD-BtuF revealed by the dynamics of complex formation

Lewinson O, Lee AT, Locher KP, Rees DC. Nat Struct Mol Biol. 2010 17(3):332-8


A P-type ATPase importer that discriminates between essential and toxic transition metals

Lewinson O, Lee AT, Rees DC. Proc Natl Acad Sci U S A. 2009 106(12):4677-82


ABC transporters: the power to change

Rees DC, Johnson E, Lewinson O. Nat Rev Mol Cell Biol. 2009 10(3):218-27


The funnel approach to the precrystallization production of membrane proteins

Lewinson O, Lee AT, Rees DC. J Mol Biol. 2008 377(1):62-73


E. coli multidrug transporter MdfA is a monomer

Sigal N, Lewinson O, Wolf SG, Bibi E. Biochemistry. 2007 46(17):5200-8


Promiscuity in multidrug recognition and transport: the bacterial MFS Mdr transporters

Lewinson O, Adler J, Sigal N, Bibi E. Mol Microbiol. 2006 (2):277-84


Do physiological roles foster persistence of drug/multidrug-efflux transporters? A case study

Krulwich TA, Lewinson O, Padan E, Bibi E. Nat Rev Microbiol. 2005 (7):566-72


Alkalitolerance: a biological function for a multidrug transporter in pH homeostasis

Lewinson O, Padan E, Bibi E. Proc Natl Acad Sci U S A. 2004 (39):14073-8


The Escherichia coli multidrug transporter MdfA catalyzes both electrogenic and electroneutral transport reactions

Lewinson O, Adler J, Poelarends GJ, Mazurkiewicz P, Driessen AJ, Bibi E. Proc Natl Acad Sci U S A. 2003; 100(4):1667-72


Role of a conserved membrane-embedded acidic residue in the multidrug transporter MdfA

Adler J, Lewinson O, Bibi E. Biochemistry. 2004 ;43(2):518-25


MdfA, an interesting model protein for studying multidrug transport

Bibi E, Adler J, Lewinson O, Edgar R. J Mol Microbiol Biotechnol. 2001 (2):171-7